Hepatoprotective effect of Nescafe in carbon tetrachloride-induced liver injury in Wistar rats

Abstract

Liver damage is of a growing concern. The study aimed at investigating the effect of Nescafe on carbon tetrachloride (CCL4)-induced liver injury. Rats were randomly grouped viz; groups: 1 to 5 of 6 rats each. Groups-2 to 5 received intraperitoneal doses of CCl4, at 2ml/Kg body weight, in 3:1 v/v preparation of CCL4 and olive oil, weekly for one month. Groups-1 and 2 were normal and positive control respectively and received distilled water (10ml/kg body water). Groups-3 to 5 were fed orally with 41.67, 62.22 and 72.22mg Nescafe suspension respectively, by gavage, weekly for one month. A day after treatment period, rats were anaesthetized and blood withdrawn from heart, using the heart puncture technique. Serum parameters viz: aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), total protein (TP), albumin, total bilirubin (TB), Total Cholesterol (T-Chol), Triglyceride, High Density Lipoprotein Cholesterol (HDL), Low density lipoprotein cholesterol (LDL) and Very Low-Density Lipoprotein cholesterol (VLDL) were studied. Group-2 activities of AST, ALT, ALP and concentrations of TB, globulin, T-Chol Triglyceride, LDL, VLDL, were increased while HDL, Albumin, TB concentrations were decreased from group-1. Aside from T-Chol and HDL, the changes were significant (P<0.05), compared to group-1. Group-3 through 5, the AST, ALT, ALP enzyme activities and globulin, TB, T-Chol, TAG, LDL VLDL concentrations decreased from Group-2. The TP, Albumin and HDL concentrations increased from group-2, ALT activities were significant (P<0.05), compared to group-1 while T-Cho and HDL were non-significant, compared to group-1. Results reviewed the protective effect of Nescafe on liver injury by CCl4.